As described in Abicht and Lochmüller (2003):

Response to acetylcholinesterase inhibitors is assessed using intravenous injection of edrophonium (Tensilon®, a fast-acting acetylcholinesterase inhibitor). An initial dose of 2 mg is injected over 15 seconds, followed by additional doses of 3 mg and 5 mg at intervals of 60 seconds, if necessary. Maximum improvement occurs within 30 seconds of the injection and persists for minutes.

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